Measuring and mitigating PCR bias in microbiota datasets

PCR amplification plays an integral role in the measurement of mixed microbial communities via high-throughput DNA sequencing of the 16S ribosomal RNA (rRNA) gene. Yet PCR is also known to introduce multiple forms of bias in 16S rRNA studies. Here we present a paired modeling and experimental approa...

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Detalhes bibliográficos
Publicado no:PLoS Comput Biol
Main Authors: Silverman, Justin D., Bloom, Rachael J., Jiang, Sharon, Durand, Heather K., Dallow, Eric, Mukherjee, Sayan, David, Lawrence A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2021
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8284789/
https://ncbi.nlm.nih.gov/pubmed/34228723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1009113
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