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Functional Evaluation of the Signal Peptides of Secreted Proteins

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Yin, Weixiao, Wang, Yufu, Chen, Tao, Lin, Yang, Luo, Chaoxi
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8275294/
https://ncbi.nlm.nih.gov/pubmed/34286044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2839
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