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Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s)

Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s...

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Dettagli Bibliografici
Pubblicato in:Bio Protoc
Autori principali: Aoki-Utsubo, Chie, Chen, Ming, Hotta, Hak
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-Protocol 2018
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8275244/
https://ncbi.nlm.nih.gov/pubmed/34286039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2830
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