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Evaluation of Firefly and Renilla Luciferase Inhibition in Reporter-Gene Assays: A Case of Isoflavonoids

Firefly luciferase is susceptible to inhibition and stabilization by compounds under investigation for biological activity and toxicity. This can lead to false-positive results in in vitro cell-based assays. However, firefly luciferase remains one of the most commonly used reporter genes. Here, we e...

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Pubblicato in:Int J Mol Sci
Autori principali: Kenda, Maša, Vegelj, Jan, Herlah, Barbara, Perdih, Andrej, Mladěnka, Přemysl, Sollner Dolenc, Marija
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI 2021
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8268740/
https://ncbi.nlm.nih.gov/pubmed/34203212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22136927
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