The Effects of Herbarium Specimen Characteristics on Short-Read NGS Sequencing Success in Nearly 8000 Specimens: Old, Degraded Samples Have Lower DNA Yields but Consistent Sequencing Success

Phylogenetic datasets are now commonly generated using short-read sequencing technologies unhampered by degraded DNA, such as that often extracted from herbarium specimens. The compatibility of these methods with herbarium specimens has precipitated an increase in broad sampling of herbarium specime...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Kates, Heather R., Doby, Joshua R., Siniscalchi, Carol M., LaFrance, Raphael, Soltis, Douglas E., Soltis, Pamela S., Guralnick, Robert P., Folk, Ryan A.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2021
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8262526/
https://ncbi.nlm.nih.gov/pubmed/34249041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2021.669064
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