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Differentiation and characterization of neural progenitors and neurons from mouse embryonic stem cells

We describe the step-by-step procedure for culturing and differentiating mouse embryonic stem cells into neuronal lineages, followed by a series of assays to characterize the differentiated cells. The E14 mouse embryonic stem cells were used to form embryoid bodies through the hanging drop method, a...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hanafiah, Aflah, Geng, Zhuangzhuang, Wang, Qiang, Gao, Zhonghua
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8256849/
https://ncbi.nlm.nih.gov/pubmed/32478755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61446
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