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Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo
We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8255939/ https://ncbi.nlm.nih.gov/pubmed/34258596 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100636 |
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