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Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii

In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed (1)H‐NMR spectroscopy using deuterium‐labeled NADH (4R‐(2)H‐NAD...

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Bibliografiske detaljer
Udgivet i:FEBS Open Bio
Main Authors: Yoneda, Kazunari, Sakuraba, Haruhiko, Araki, Tomohiro, Ohshima, Toshihisa
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8255831/
https://ncbi.nlm.nih.gov/pubmed/34043290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/2211-5463.13200
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