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Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii

In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed (1)H‐NMR spectroscopy using deuterium‐labeled NADH (4R‐(2)H‐NAD...

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Dades bibliogràfiques
Publicat a:FEBS Open Bio
Autors principals: Yoneda, Kazunari, Sakuraba, Haruhiko, Araki, Tomohiro, Ohshima, Toshihisa
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2021
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8255831/
https://ncbi.nlm.nih.gov/pubmed/34043290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/2211-5463.13200
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