Paramagnetic Solid‐State NMR to Localize the Metal‐Ion Cofactor in an Oligomeric DnaB Helicase

Paramagnetic metal ions can be inserted into ATP‐fueled motor proteins by exchanging the diamagnetic Mg(2+) cofactor with Mn(2+) or Co(2+). Then, paramagnetic relaxation enhancement (PRE) or pseudo‐contact shifts (PCSs) can be measured to report on the localization of the metal ion within the protei...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Chemistry
Prif Awduron: Zehnder, Johannes, Cadalbert, Riccardo, Terradot, Laurent, Ernst, Matthias, Böckmann, Anja, Güntert, Peter, Meier, Beat H., Wiegand, Thomas
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: John Wiley and Sons Inc. 2021
Pynciau:
Full Papers
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8252064/
https://ncbi.nlm.nih.gov/pubmed/33822417
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202100462
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