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Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics
Microscale thermophoresis (MST) is a versatile technique to measure binding affinities of binder–ligand systems, based on the directional movement of molecules in a temperature gradient. We extended MST to measure binding kinetics as well as binding affinity in a single experiment by increasing the...
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| Veröffentlicht in: | Angew Chem Int Ed Engl |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8251828/ https://ncbi.nlm.nih.gov/pubmed/33793031 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202101261 |
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