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Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics

Microscale thermophoresis (MST) is a versatile technique to measure binding affinities of binder–ligand systems, based on the directional movement of molecules in a temperature gradient. We extended MST to measure binding kinetics as well as binding affinity in a single experiment by increasing the...

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Vydáno v:Angew Chem Int Ed Engl
Hlavní autoři: Stein, Julian A. C., Ianeselli, Alan, Braun, Dieter
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8251828/
https://ncbi.nlm.nih.gov/pubmed/33793031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202101261
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