Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by...

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Bibliographic Details
Published in:Chem Sci
Main Authors: Kim, Do-Hyeon, Chang, Yeonho, Park, Soyeon, Jeong, Min Gyu, Kwon, Yonghoon, Zhou, Kai, Noh, Jungeun, Choi, Yun-Kyu, Hong, Triet Minh, Chang, Young-Tae, Ryu, Sung Ho
Format: Artigo
Language:Inglês
Published: The Royal Society of Chemistry 2021
Subjects:
Chemistry
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8246296/
https://ncbi.nlm.nih.gov/pubmed/34257864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d1sc00612f
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