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Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy

A computational method was developed to recover the three-dimensional coordinates of gold nanoparticles specifically attached to a protein complex from tilt-pair images collected by electron microscopy. The program was tested on a simulated dataset and applied to a real dataset comprising tilt-pair...

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Detalhes bibliográficos
Publicado no:Ultramicroscopy
Main Authors: Jagota, Milind, Townshend, Raphael J.L., Kang, Lin-Woo, Bushnell, David A., Dror, Ron O., Kornberg, Roger D., Azubel, Maia
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8238918/
https://ncbi.nlm.nih.gov/pubmed/34062386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ultramic.2021.113302
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