Benchmarking DNA Extraction Methods for Phylogenomic Analysis of Sub-Antarctic Rhodococcus and Williamsia Species

Bacteria containing mycolic acids in their cell envelope are often recalcitrant to cell lysis, so extracting DNA of sufficient quality for third-generation sequencing and high-fidelity genome assembly requires optimization, even when using commercial kits with protocols for hard-to-lyse bacteria. We...

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Udgivet i:Microorganisms
Main Authors: Nahar, Akhikun, Baker, Anthony L., Nichols, David S., Bowman, John P., Britz, Margaret L.
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8227252/
https://ncbi.nlm.nih.gov/pubmed/34207615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/microorganisms9061253
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