High-precision targeting workflow for volume electron microscopy

Cells are 3D objects. Therefore, volume EM (vEM) is often crucial for correct interpretation of ultrastructural data. Today, scanning EM (SEM) methods such as focused ion beam (FIB)–SEM are frequently used for vEM analyses. While they allow automated data acquisition, precise targeting of volumes of...

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Détails bibliographiques
Publié dans:J Cell Biol
Auteurs principaux: Ronchi, Paolo, Mizzon, Giulia, Machado, Pedro, D’Imprima, Edoardo, Best, Benedikt T., Cassella, Lucia, Schnorrenberg, Sebastian, Montero, Marta G., Jechlinger, Martin, Ephrussi, Anne, Leptin, Maria, Mahamid, Julia, Schwab, Yannick
Format: Artigo
Langue:Inglês
Publié: Rockefeller University Press 2021
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8225610/
https://ncbi.nlm.nih.gov/pubmed/34160561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.202104069
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