Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division

Quantification of cellular proliferation in humans is important for understanding biology and responses to injury and disease. However, existing methods require administration of tracers that cannot be ethically administered in humans. We present a protocol for the direct quantification of cellular...

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Udgivet i:Nat Protoc
Main Authors: Yester, Jessie W., Liu, Honghai, Gyngard, Frank, Ammanamanchi, Niyatie, Little, Kathryn C., Thomas, Dawn, Sullivan, Mara L. G., Lal, Sean, Steinhauser, Matthew L., Kühn, Bernhard
Format: Artigo
Sprog:Inglês
Udgivet: 2021
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8221415/
https://ncbi.nlm.nih.gov/pubmed/33627842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-00477-y
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