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Preculture in an enriched nutrient medium greatly enhances the Agrobacterium-mediated transformation efficiency in Arabidopsis T87 cultured cells

The Arabidopsis T87 cell line has been widely used in both basic and biotechnological plant sciences. Agrobacterium-mediated transformation of this cell line was reported to be highly efficient when precultured in Gamborg’s B5 medium for a few days. However, because we could not obtain the expected...

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Vydáno v:Plant Biotechnol (Tokyo)
Hlavní autoři: Hata, Takayuki, Mukae, Kazuki, Satoh, Soichrou, Matsuo, Mitsuhiro, Obokata, Junichi
Médium: Artigo
Jazyk:Inglês
Vydáno: Japanese Society for Plant Cell and Molecular Biology 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8215453/
https://ncbi.nlm.nih.gov/pubmed/34177340
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5511/plantbiotechnology.20.1211b
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