Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study host–microbe cross talk

Reproducible in vivo models are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characte...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Steimle, Alex, De Sciscio, Alessandro, Neumann, Mareike, Grant, Erica T., Pereira, Gabriel V., Ohno, Hiroshi, Martens, Eric C., Desai, Mahesh S.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8209845/
https://ncbi.nlm.nih.gov/pubmed/34179836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100607
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