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Simple, Inexpensive RNA Isolation and One‐Step RT‐qPCR Methods for SARS‐CoV‐2 Detection and General Use

The most common method for RNA detection involves reverse transcription followed by quantitative polymerase chain reaction (RT‐qPCR) analysis. Commercial one‐step master mixes—which include both a reverse transcriptase and a thermostable polymerase and thus allow performing both the RT and qPCR step...

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Bibliografski detalji
Izdano u:Curr Protoc
Glavni autori: Graham, Thomas G.W., Dugast‐Darzacq, Claire, Dailey, Gina M., Darzacq, Xavier, Tjian, Robert
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2021
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8206771/
https://ncbi.nlm.nih.gov/pubmed/33905620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.130
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