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Investigating Localization of Chimeric Transporter Proteins within Chloroplasts of Arabidopsis thaliana

In this protocol, we describe a method to design chimeric proteins for specific targeting to the inner envelope membrane (IEM) of Arabidopsis chloroplasts and the confirmation of their localization by biochemical analysis. Specific targeting to the chloroplast IEM can be achieved by fusing the prote...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Uehara, Susumu, Ito-Inaba, Yasuko, Inaba, Takehito
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203966/
https://ncbi.nlm.nih.gov/pubmed/34179258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2723
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