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Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method forVisual Detection of Protein-protein Interactions
The visible immunoprecipitation (VIP) assay is a convenient alternative to conventional co-immunoprecipitation ( Katoh et al., 2015 ). By processing lysates from cells co-expressing GFP-fusion and RFP-fusion proteins for immunoprecipitation with GST-tagged anti-GFP Nanobody and glutathione-Sepharose...
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| Izdano u: | Bio Protoc |
|---|---|
| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Bio-Protocol
2018
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8203940/ https://ncbi.nlm.nih.gov/pubmed/34179269 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2687 |
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