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Culture and Nucleofection of Postnatal Day 7 Cortical and Cerebellar Mouse Astroglial Cells

Lineage reprogramming of astroglial cells isolated from different brain regions leads to the generation of different neuronal subtypes. This protocol describes the isolation and culture of neocortical and cerebellar astrocytes from postnatal mice. We also present a comprehensive description of the m...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Chouchane, Malek, Costa, Marcos Romualdo
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203934/
https://ncbi.nlm.nih.gov/pubmed/34179254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2712
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