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Visualization of RNA 3’ ends in Escherichia coli Using 3’ RACE Combined with Primer Extension
In this assay, 3’ RACE (Rapid Amplification of cDNA 3’ Ends) followed by PE (primer extension), abbreviated as 3’ RACE-PE is used to identify the mRNA 3’ ends. The following protocol describes the amplification of the mRNA 3’ ends at the galactose operon in E. coli and the corresponding visualizatio...
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| Publicado no: | Bio Protoc |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8203919/ https://ncbi.nlm.nih.gov/pubmed/34179278 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2752 |
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