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Visualization of RNA 3’ ends in Escherichia coli Using 3’ RACE Combined with Primer Extension

In this assay, 3’ RACE (Rapid Amplification of cDNA 3’ Ends) followed by PE (primer extension), abbreviated as 3’ RACE-PE is used to identify the mRNA 3’ ends. The following protocol describes the amplification of the mRNA 3’ ends at the galactose operon in E. coli and the corresponding visualizatio...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Wang, Xun, Jeon, Heung Jin, N, Monford Paul Abishek, He, Jin, Lim, Heon M.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203919/
https://ncbi.nlm.nih.gov/pubmed/34179278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2752
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