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Visualization of RNA 3’ ends in Escherichia coli Using 3’ RACE Combined with Primer Extension

In this assay, 3’ RACE (Rapid Amplification of cDNA 3’ Ends) followed by PE (primer extension), abbreviated as 3’ RACE-PE is used to identify the mRNA 3’ ends. The following protocol describes the amplification of the mRNA 3’ ends at the galactose operon in E. coli and the corresponding visualizatio...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Wang, Xun, Jeon, Heung Jin, N, Monford Paul Abishek, He, Jin, Lim, Heon M.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203919/
https://ncbi.nlm.nih.gov/pubmed/34179278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2752
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