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Organotypic culture assays for murine and human primary and metastatic-site tumors

Cancer invasion and metastasis are challenging to study in vivo since they occur deep inside the body over extended time periods. Organotypic 3D culture of fresh tumor tissue enables convenient real-time imaging, genetic and microenvironmental manipulation, and molecular analysis. Here we provide de...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Padmanaban, Veena, Grasset, Eloise M., Neumann, Neil M., Fraser, Andrew K., Henriet, Elodie, Matsui, William, Tran, Phuoc T., Cheung, Kevin J., Georgess, Dan, Ewald, Andrew J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8202162/
https://ncbi.nlm.nih.gov/pubmed/32690957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0335-3
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