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Calculation of the Protein Turnover Rate Using the Number of Incorporated (2)H Atoms and Proteomics Analysis of a Single Labelled Sample.
Rate constant estimation with heavy water requires a long-term experiment with data collection at multiple time points (3-4 weeks for mitochondrial proteome dynamics in mice and much longer in other species). When tissue proteins are analyzed, this approach requires euthanizing animals at each time...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8201887/ https://ncbi.nlm.nih.gov/pubmed/31638786 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b02757 |
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