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Expanding the SiMPl Plasmid Toolbox for Use with Spectinomycin/Streptomycin

[Image: see text] We recently developed the SiMPl plasmid toolbox, which is constituted by pairs of plasmids, generically indicated as pSiMPl(x)_N and pSiMPl(x)_C, which can be stably maintained in Escherichia coli with a single antibiotic x. The method exploits the split intein gp41–1 to reconstitu...

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Detalles Bibliográficos
Publicado en:ACS Omega
Main Authors: Palanisamy, Navaneethan, Ballestin, Jara Ballestin, Di Ventura, Barbara
Formato: Artigo
Idioma:Inglês
Publicado: American Chemical Society 2021
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8190784/
https://ncbi.nlm.nih.gov/pubmed/34124437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.1c00649
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