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Generation of locus-specific degradable tag knock-ins in mouse and human cell lines
Protein degradation technologies represent a powerful functional genomics tool, allowing fast and controllable target protein depletion. Establishing these systems requires a knock-in of the degradation tag into both endogenous target gene alleles. Here, we provide a step-by-step protocol for the ef...
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| Publicado en: | STAR Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8188616/ https://ncbi.nlm.nih.gov/pubmed/34151298 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100575 |
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