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A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride

The visualization of chloride in living cells with fluorescent sensors is linked to our ability to design hosts that can overcome the energetic penalty of desolvation to bind chloride in water. Fluorescent proteins can be used as biological supramolecular hosts to address this fundamental challenge....

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Publicado en:Chem Sci
Autores principales: Tutol, Jasmine N., Lee, Jessica, Chi, Hsichuan, Faizuddin, Farah N., Abeyrathna, Sameera S., Zhou, Qin, Morcos, Faruck, Meloni, Gabriele, Dodani, Sheel C.
Formato: Artigo
Lenguaje:Inglês
Publicado: The Royal Society of Chemistry 2021
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8179538/
https://ncbi.nlm.nih.gov/pubmed/34163777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc06061e
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