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Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay
Herein, we describe an isothermal proximity CRISPR Cas12a assay that harnesses the target-induced indiscrimitive single-stranded DNase activity of Cas12a for the quantitative profiling of gene expression at the mRNA level and detection of proteins with high sensitivity and specificity. The target re...
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| Publicado en: | Chem Sci |
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| Autores principales: | , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
The Royal Society of Chemistry
2021
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8179305/ https://ncbi.nlm.nih.gov/pubmed/34163977 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc06113a |
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