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Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca

We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation. Eight different primer pairs targeting different variab...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Darwish, Nadia, Shao, Jonathan, Schreier, Lori L., Proszkowiec-Weglarz, Monika
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8178357/
https://ncbi.nlm.nih.gov/pubmed/34088939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-91387-w
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