Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2

To increase the repertoire of PCR based laboratory developed tests (LDTs) for the detection of SARS-CoV-2, we describe a new multiplex assay (SORP), targeting the SARS-CoV-2's, Spike and ORF8 genes. The widely used human RNaseP internal control was modified to specifically co-amplify the RNaseP...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cell Probes
Hauptverfasser: Gadkar, Vijay J., Goldfarb, David M., Young, Virginia, Watson, Nicole, Al-Rawahi, Ghada N., Srigley, Jocelyn A., Tilley, Peter
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier Ltd. 2021
Schlagworte:
Original Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8176879/
https://ncbi.nlm.nih.gov/pubmed/34089805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2021.101744
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