Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts

Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Yasuda, Tadahito, Koiwa, Mayu, Yonemura, Atsuko, Akiyama, Takahiko, Baba, Hideo, Ishimoto, Takatsugu
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8176369/
https://ncbi.nlm.nih.gov/pubmed/34136831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100553
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