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Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number

Recently, in food safety and various other fields, qualitative and quantitative gene analysis using real-time polymerase chain reaction (PCR) method has become increasingly popular. The limit of detection (LOD) and quantifiable range for these measurements depends on the range and precision of DNA c...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ki, Unoh, Suzuki, Takeru, Nakazawa, Satoshi, Yonekawa, Yuuki, Watanabe, Kazuki, Hashimoto, Michie, Hatada, Shigeo, Unno, Hirotaka
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8172552/
https://ncbi.nlm.nih.gov/pubmed/34078977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-90959-0
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