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Electroporation of Mouse Follicles, Oocytes and Embryos without Manipulating Zona Pellucida

Electroporation is an effective technique of transfection, but its efficiency depends on the optimization of various parameters. In this study, a simplified and efficient method of gene manipulation was standardized through electroporation to introduce a recombinant green fluorescent protein (GFP) c...

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Detalhes bibliográficos
Publicado no:J Dev Biol
Main Authors: Hakim, Bilal Ahmad, Tyagi, Vaishali, Agnihotri, Saurabh Kumar, Nath, Amar, Agrawal, Ankit Kumar, Jain, Ankita, Singh, Deependra, Konwar, Rituraj, Sachdev, Monika
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8167572/
https://ncbi.nlm.nih.gov/pubmed/33915920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/jdb9020013
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