Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design

For the whole GFP family, a few cases, when a single mutation in the chromophore environment strongly inhibits maturation, were described. Here we study EYFP-F165G – a variant of the enhanced yellow fluorescent protein – obtained by a single F165G replacement, and demonstrated multiple fluorescent s...

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Detalhes bibliográficos
Publicado no:Comput Struct Biotechnol J
Main Authors: Pletneva, Nadya V., Maksimov, Eugene G., Protasova, Elena A., Mamontova, Anastasia V., Simonyan, Tatiana R., Ziganshin, Rustam H., Lukyanov, Konstantin A., Muslinkina, Liya, Pletnev, Sergei, Bogdanov, Alexey M., Pletnev, Vladimir Z.
Formato: Artigo
Idioma:Inglês
Publicado em: Research Network of Computational and Structural Biotechnology 2021
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8163865/
https://ncbi.nlm.nih.gov/pubmed/34136094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.csbj.2021.05.017
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