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SDS-induced multi-stage unfolding of a small globular protein through different denatured states revealed by single-molecule fluorescence

Ionic surfactants such as sodium dodecyl sulfate (SDS) unfold proteins in a much more diverse yet effective way than chemical denaturants such as guanidium chloride (GdmCl). But how these unfolding processes compare on a molecular level is poorly understood. Here, we address this question by scrutin...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Krainer, Georg, Hartmann, Andreas, Bogatyr, Vadim, Nielsen, Janni, Schlierf, Michael, Otzen, Daniel E.
Formato: Artigo
Idioma:Inglês
Publicado em: The Royal Society of Chemistry 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8163379/
https://ncbi.nlm.nih.gov/pubmed/34123163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc02100h
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