Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH

[Image: see text] Isobaric labeling via tandem mass tag (TMT) reagents enables sample multiplexing prior to LC–MS/MS, facilitating high-throughput large-scale quantitative proteomics. Consistent and efficient labeling reactions are essential to achieve robust quantification; therefore, embedded in o...

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Detalhes bibliográficos
Publicado no:ACS Omega
Main Authors: Hutchinson-Bunch, Chelsea, Sanford, James A., Hansen, Joshua R., Gritsenko, Marina A., Rodland, Karin D., Piehowski, Paul D., Qian, Wei-Jun, Adkins, Joshua N.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2021
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8154127/
https://ncbi.nlm.nih.gov/pubmed/34056417
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.1c00776
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