Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system

Environmental stimuli evoke transient increases of the cytosolic Ca(2+) level. To identify upstream components of Ca(2+) signaling, we have optimized two forward genetic screening systems based on Ca(2+) reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS...

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Bibliographische Detailangaben
Veröffentlicht in:STAR Protoc
Hauptverfasser: Sun, Shujing, Zhang, Xiaoyan, Chen, Kong, Zhu, Xiaohong, Zhao, Yang
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021
Schlagworte:
Protocol
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8144734/
https://ncbi.nlm.nih.gov/pubmed/34041505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100558
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