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High-throughput live imaging of microcolonies to measure heterogeneity in growth and gene expression

Precise measurements of between- and within-strain heterogeneity in microbial growth rates are essential for understanding genetic and environmental inputs into stress tolerance, pathogenicity, and other key components of fitness. This manuscript describes a microscope-based assay that tracks approx...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Sartori, Federica M. O., Buzby, Cassandra, Plavskin, Yevgeniy, Siegal, Mark L.
Format: Artigo
Jezik:Inglês
Izdano: 2021
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8142558/
https://ncbi.nlm.nih.gov/pubmed/33938878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/62038
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