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Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms

MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a stre...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: McKnight, Laura E., Crandall, Johnathan G., Bailey, Thomas B., Banks, Orion G.B., Orlandi, Kona N., Truong, Vi N., Donovan, Drake A., Waddell, Grace L., Wiles, Elizabeth T., Hansen, Scott D., Selker, Eric U., McKnight, Jeffrey N.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8141940/
https://ncbi.nlm.nih.gov/pubmed/34041500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100486
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