An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. However, cleavage of the amplicons and primers by the cis- and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reactio...

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Detalhes bibliográficos
Publicado no:Bioact Mater
Main Authors: Gong, Jiaojiao, Kan, Lijuan, Zhang, Xiuming, He, Ying, Pan, Jiaqiang, Zhao, Liping, Li, Qianyun, Liu, Menghao, Tian, Jie, Lin, Sili, Lu, Zhouyu, Xue, Liang, Wang, Chaojun, Tang, Guanghui
Formato: Artigo
Idioma:Inglês
Publicado em: KeAi Publishing 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8141609/
https://ncbi.nlm.nih.gov/pubmed/34095617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bioactmat.2021.05.005
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