Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a protocol for...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Guldner, Ian H., Golomb, Samantha M., Wang, Qingfei, Wang, Emilia, Zhang, Siyuan
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8138863/
https://ncbi.nlm.nih.gov/pubmed/34036283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100537
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