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Molecular structure, DNA binding mode, photophysical properties and recommendations for use of SYBR Gold

SYBR Gold is a commonly used and particularly bright fluorescent DNA stain, however, its chemical structure is unknown and its binding mode to DNA remains controversial. Here, we solve the structure of SYBR Gold by NMR and mass spectrometry to be [2-(4-{[diethyl(methyl)ammonio]methyl}phenyl)-6-metho...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Kolbeck, Pauline J, Vanderlinden, Willem, Gemmecker, Gerd, Gebhardt, Christian, Lehmann, Martin, Lak, Aidin, Nicolaus, Thomas, Cordes, Thorben, Lipfert, Jan
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8136779/
https://ncbi.nlm.nih.gov/pubmed/33905507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab265
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