HCK and ABAA: A Newly Designed Pipeline to Improve Fungi Metabarcoding Analysis

INTRODUCTION: The fungi ITS sequence length dissimilarity, non-specific amplicons, including chimaera formed during Polymerase Chain Reaction (PCR), added to sequencing errors, create bias during similarity clustering and abundance estimation in the downstream analysis. To overcome these challenges,...

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Veröffentlicht in:Front Microbiol
Hauptverfasser: Mlaga, Kodjovi D., Mathieu, Alban, Beauparlant, Charles Joly, Ott, Alban, Khodr, Ahmad, Perin, Olivier, Droit, Arnaud
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2021
Schlagworte:
Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8134036/
https://ncbi.nlm.nih.gov/pubmed/34025601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2021.640693
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