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Intestinal epithelial cell monolayer culture and multiplex macromolecular permeability assay for in vitro analysis of tight junction size-selectivity
Tight junctions form a selectively-permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ~8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway ac...
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| Published in: | Curr Protoc Immunol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8122669/ https://ncbi.nlm.nih.gov/pubmed/33175441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.112 |
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