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Intestinal epithelial cell monolayer culture and multiplex macromolecular permeability assay for in vitro analysis of tight junction size-selectivity

Tight junctions form a selectively-permeable barrier that limits paracellular flux across epithelial-lined surfaces. Small molecules (less than ~8 Å diameter) can traverse the junction via the size- and charge-selective, high-conductance pore pathway. In contrast, the low-conductance leak pathway ac...

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Podrobná bibliografie
Vydáno v:Curr Protoc Immunol
Hlavní autoři: Pongkorpsakol, Pawin, Turner, Jerrold R., Zuo, Li
Médium: Artigo
Jazyk:Inglês
Vydáno: 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8122669/
https://ncbi.nlm.nih.gov/pubmed/33175441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.112
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