Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them....

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Библиографические подробности
Опубликовано в: :STAR Protoc
Главные авторы: Calvo-Garrido, Javier, Winn, Dania, Maffezzini, Camilla, Wedell, Anna, Freyer, Christoph, Falk, Anna, Wredenberg, Anna
Формат: Artigo
Язык:Inglês
Опубликовано: Elsevier 2021
Предметы:
Protocol
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC8121988/
https://ncbi.nlm.nih.gov/pubmed/34027486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100528
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