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Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genom...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8113982/ https://ncbi.nlm.nih.gov/pubmed/34013210 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100514 |
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