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Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as...
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| Publicado no: | STAR Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8102171/ https://ncbi.nlm.nih.gov/pubmed/33997814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100500 |
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