Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

Thermophilic organisms are extensively studied in industrial biotechnology, for exploration of the limits of life, and in other contexts. Their optimal growth at high temperatures presents a challenge for the development of genetic tools for their genome editing, since genetic markers and selection...

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Pubblicato in:Sci Rep
Autori principali: Adalsteinsson, Bjorn Thor, Kristjansdottir, Thordis, Merre, William, Helleux, Alexandra, Dusaucy, Julia, Tourigny, Mathilde, Fridjonsson, Olafur, Hreggvidsson, Gudmundur Oli
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2021
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8100143/
https://ncbi.nlm.nih.gov/pubmed/33953310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-89029-2
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