Nearest-neighbour transition-state analysis for nucleic acid kinetics

We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters for association and dissociation. Reactions were performed in 1.0 M NaCl (for literature comparisons) and 2.2 mM MgCl(2) (PCR conditions). Dissociatio...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Rejali, Nick A, Ye, Felix D, Zuiter, Aisha M, Keller, Caroline C, Wittwer, Carl T
Format: Artigo
Language:Inglês
Published: Oxford University Press 2021
Subjects:
Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8096236/
https://ncbi.nlm.nih.gov/pubmed/33823552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab205
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