Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads (SSMBs)

Plasmid DNA (pDNA) isolation from bacterial cells is one of the most common and critical steps in molecular cloning and biomedical research. Almost all pDNA purification involves disruption of bacteria, removal of membrane lipids, proteins and genomic DNA, purification of pDNA from bulk lysate, and...

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Vydáno v:Genes Dis
Hlavní autoři: Wang, Xi, Zhao, Ling, Wu, Xiaoxing, Luo, Huaxiu, Wu, Di, Zhang, Meng, Zhang, Jing, Pakvasa, Mikhail, Wagstaff, William, He, Fang, Mao, Yukun, Zhang, Yongtao, Niu, Changchun, Wu, Meng, Zhao, Xia, Wang, Hao, Huang, Linjuan, Shi, Deyao, Liu, Qing, Ni, Na, Fu, Kai, Hynes, Kelly, Strelzow, Jason, El Dafrawy, Mostafa, He, Tong-Chuan, Qi, Hongbo, Zeng, Zongyue
Médium: Artigo
Jazyk:Inglês
Vydáno: Chongqing Medical University 2020
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8093646/
https://ncbi.nlm.nih.gov/pubmed/33997177
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gendis.2020.04.013
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